14-440-M

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

• Codice UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in E. coli; expressed in BL21 DE3 cells
• Nome Commerciale: Upstate^®
• Forma fisica: liquid
• Attività specifica: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.); 8580 U/mg
• Durata: 1 yr
• PM: M_w 40.7 kDa
• Purificato mediante: chromatography
• Reattività contro le specie: human
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable (kinase)
• Solubilità: water: soluble
• Numero d’accesso: (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. ); NM_002730.3
• N° accesso NCBI: NM_002730.3; NM_207518.1
• N° accesso UniProt: P17612
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PRKACA(46909581)

Descrizione

Descrizione generale

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.

Recombinant human full length PKA, catalytic subunit type alpha, untagged

Azioni biochim/fisiol

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Confezionamento

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Qualità

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Descrizione del bersaglio

PKA

Linkage

Replaces: 14-114

Stato fisico

Phosphocellulose P11 chromatography

Stoccaggio e stabilità

1 year at -70°C

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1