P5813

Anti-p53 antibody, Mouse monoclonal

clone BP53-12, ascites fluid

• Sinonimo/i: Anti-TRP53, Anti-Transformation-related protein 53
• Numero MDL: MFCD00164709
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: BP53-12, monoclonal
• PM: antigen 53 kDa
• contiene: 15 mM sodium azide
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein
• Isotipo: IgG2a
• N° accesso UniProt: P04637
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TP53(7157)

Descrizione

Descrizione generale

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

Immunogeno

recombinant human wild-type p53 protein.

Applicazioni

Monoclonal Anti-p53 antibody produced in mouse has been used in:

• western blotting
• immunohistochemistry
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

Azioni biochim/fisiol

The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable