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P4056

Sigma-Aldrich

Platelet-Derived Growth Factor-BB from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinonimo/i:

PDGF-BB

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About This Item

Numero MDL:
MFCD01094933
Codice UNSPSC:
12352202
NACRES:
NA.77

Nome del prodotto

Platelet-Derived Growth Factor-BB from rat, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Ricombinante

expressed in E. coli

Livello qualitativo

100

Stato

lyophilized powder

Potenza

1.62 ng/mL EC50

Qualità

endotoxin tested

PM

~30 kDa

Confezionamento

pkg of 5x50 μg
pkg of 50 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

Impurezze

≤1 EU/μg

N° accesso UniProt

P31240

Temperatura di conservazione

−20°C

Informazioni sul gene

rat ... Pdgfb(24628)

Categorie correlate

Descrizione generale

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Applicazioni

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Azioni biochim/fisiol

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Altre note

Dimer of the B chain of rat PDGF.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Risultati analitici

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCB9745
SLBV9077
SLBR5692V
SLBP3684V
SLBJ4664V

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Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
Millette E, et al.
Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
Xin Zhou et al.
Current protocols, 2(5), e416-e416 (2022-05-10)
The serine/threonine protein kinase Akt integrates diverse upstream inputs to regulate cell survival, growth, metabolism, migration, and differentiation. Mounting evidence suggests that Akt activity is differentially regulated depending on its subcellular location, which can include the plasma membrane, endomembrane, and
Skeletal muscle tissue engineering: A maturation model promoting long-term survival of myotubes, structural development of the excitation?contraction coupling apparatus and neonatal myosin heavy chain expression.
Das M, et al.
Biomaterials, 30.29, 5392-5402 (2009)
Role of platelets in the development of atherosclerosis.
Quintana L, et al.
Tissue Engineering: Part A, 15.1, 45-54 (2008)
Eileen M Bauer et al.
PloS one, 9(5), e96720-e96720 (2014-05-20)
Pulmonary hypertension (PH) is a progressive and fatal disease with no cure. Vascular remodeling in PH involves intraluminal growth of endothelial and smooth muscle cells, leading to obliterative vascular lesions. Cell growth in these lesions is quasi-neoplastic, with evidence of
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Articoli

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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