OGS589
PBR322 - PBR322 LOW COPY CLONING VECTOR
plasmid vector for molecular cloning
Sinonimo/i:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.85
Prodotti consigliati
Forma fisica
buffered aqueous solution
PM
size 4361 bp
Selezione batterica
ampicillin
Origine di replicazione
BR322 (15 copies)
Clivaggio proteico
no cleavage
Gene reporter
none
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
Descrizione generale
This plasmid contains the BR322 (low copy number) origin of replication and provides approximately 20 plasmid copies per bacterial cell. It also contains the mammalian CMV promoter upstream of the MCS and Kan resistance for selection of transfected mammalian cells. Low copy number plasmids are useful for very large transgenes or for transgenes that can be toxic to bacterial cells.
Promoter Expression Level:
Promoter Expression Level:
Applicazioni
BR322 Information: BR322 is a wild type plasmid isolated from E.coli. It contains a low copy origin that allows for plasmid maintenance at a frequency of approximately 10-100 plasmid copies per cell. Typically this number is closer to 30-40 copies per cell. Most common cloning plasmids contain a derivative of the origin of replication in this plasmid called pUC. The pUC origin was created by removing the Rep repressor protein that normally regulates plasmid copy number in E.coli and also making a single point mutation in the origin of replication itself. The point mutation is the most important difference between the origin of replication.This plasmid can be used as a general purpose low copy cloning vector.
Sequenza
To view sequence information for this product, please visit the product page
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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