OGS1879
PSF-TEF1-NH2-INTS - INVERTASE SECRETION PLASMID
plasmid vector for molecular cloning
Sinonimo/i:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.85
Forma fisica
buffered aqueous solution
PM
size 6787 bp
Selezione batterica
kanamycin
Origine di replicazione
2Micron
pUC (500 copies)
Clivaggio proteico
no cleavage
Posizione del tag peptidico
N-terminal
Promotore
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
Gene reporter
none
Segnale di secrezione
alpha factor FL
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
Selezione lieviti
uracil
Descrizione generale
This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
Sequenza
To view sequence information for this product, please visit the product page
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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