Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

NA0800

Sigma-Aldrich

GenElute HP Select Plasmid Gigaprep Kit

greener alternative

sufficient for 5 preparations

Sinonimo/i:

HP gigaprep kit, endo free gigaprep kit, endotoxin free giga kit, endotoxin free plasmid kit, fast gigaprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation, Gen Elute, GenElute HP Plasmid Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 5 preparations

Livello qualitativo

Caratteristiche più verdi

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

tecniche

DNA extraction: suitable

Categoria alternativa più verde

Temperatura di conservazione

15-25°C

Descrizione generale

The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.

The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Applicazioni

GenElute® HP Select Plasmid Gigaprep Kit has been used to purify plasmids.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research.

Caratteristiche e vantaggi

  • Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
  • Only 90 minutes from pelleted cells to purified plasmid
  • Vacuum format with no ethanol precipitation
  • Can purify low-, medium-, and high-copy plasmid DNA

Altre note

For additional information, please see www.sigma-aldrich.com/genelutehp.

Note legali

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • G3671GenElute HP Pro Gigaprep Binding ColumnSDS

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

Organi bersaglio

Central nervous system, Lungs

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

77.0 °F - closed cup

Punto d’infiammabilità (°C)

25 °C - closed cup


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Plasmid DNA-coding p62 as a bone effective anti-inflammatory/anabolic agent.
Sabbieti MG
Oncotarget, 6(6), 3590-3599 (2015)
V D Boppana et al.
Parasite immunology, 31(6), 287-295 (2009-06-06)
Mosquitoes represent the most important vector for transmitting pathogens that cause human disease. Central to pathogen transmission is the ability to divert the host immune system away from Th1 and towards Th2 responsiveness. Identification of the mosquito factor(s) critical for

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.