NA0410
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
sufficient for 25 preparations
Sinonimo/i:
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
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About This Item
Codice UNSPSC:
41105501
NACRES:
NA.52
Prodotti consigliati
impiego
sufficient for 25 preparations
Caratteristiche più verdi
Designing Safer Chemicals
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tecniche
DNA extraction: suitable
Categoria alternativa più verde
Temperatura di conservazione
15-25°C
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Descrizione generale
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
Applicazioni
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit has been used in the isolation of plasmid from swine liver and from competent Escherichia coli cells.
Caratteristiche e vantaggi
- Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
- Only 35 minutes from pelleted cells to purified plasmid
- Convenient vacuum format
Principio
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.
Altre note
For additional information, please see www.sigma-aldrich.com/genelutehp.
Note legali
GenElute is a trademark of Sigma-Aldrich Co. LLC
Prodotti correlati
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Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Central nervous system
Codice della classe di stoccaggio
3 - Flammable liquids
Punto d’infiammabilità (°F)
77.0 °F - closed cup
Punto d’infiammabilità (°C)
25 °C - closed cup
Certificati d'analisi (COA)
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