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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Sinonimo/i:

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Formula condensata:
O2NC6H4OP(O)(ONa)2
Numero CAS:
4264-83-9
Peso molecolare:
263.05
Numero MDL:
MFCD00007319
Codice UNSPSC:
12352204
ID PubChem:
24897835
NACRES:
NA.83

product name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Forma fisica

liquid

Livello qualitativo

200

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Stringa SMILE

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
VIYFPAMJCJLZKD-UHFFFAOYSA-L

Descrizione generale

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Applicazioni

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Azioni biochim/fisiol

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Avvertenza

Stable at least one year when stored below 0 °C.

Stato fisico

Ready-to-use.

Pittogrammi

Health hazardCorrosion
GHS08,GHS05

Avvertenze

Danger

Indicazioni di pericolo

H290,H315,H318,H361fd,H373

Classi di pericolo

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Organi bersaglio

Kidney,Liver,Blood

Codice della classe di stoccaggio

8B - Non-combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen® Custom Peptide Library

Review a high-throughput ELISA-based method to identify peptide substrates for class-specific and/or enzyme-specific Protein Tyrosine Kinases (PTKs) that can be utilized for the detection of kinase activity both in vivo and in vitro.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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