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N2415

Sigma-Aldrich

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

Sinonimo/i:

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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About This Item

Numero CAS:
9030-21-1
Classificazione EC (Enzyme Commission):
2.4.2.1 (BRENDA, IUBMB)
Numero CE:
232-857-3
Numero MDL:
MFCD00131739
Codice UNSPSC:
12352204

Ricombinante

expressed in E. coli

Livello qualitativo

200

Saggio

≥80% protein basis (biuret)

Forma fisica

lyophilized powder

Attività specifica

≥10 units/mg protein

Nota sulla sequenza

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

N° accesso UniProt

P0ABP8

Temperatura di conservazione

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
MRWXACSTFXYYMV-FDDDBJFASA-N

Informazioni sul gene

Escherichia coli ... deoD(945654)

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Applicazioni

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Azioni biochim/fisiol

Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate

Definizione di unità

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Nota sulla preparazione

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Primary T-cell immunodeficiencies
Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)
Disorders of purines and pyrimidines
Handbook of Clinical Neurology, 827-838 (2014)
Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Johnston S B and Raines R T
Biochemistry, 54(7), 1576-1582 (2015)
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Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and
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PloS one, 10(1), e0116898-e0116898 (2015-01-22)
Phosphatase and tensin homologue deleted from chromosome ten (PTEN) is a lipid phosphatase tumor suppressor that is lost or inactivated in most human tumors. The enzyme catalyzes the hydrolysis of phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) to form phosphatidylinositol-(4,5)-bisphosphate (PIP2) and inorganic phosphate. Here
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