A5168
Adenosine Deaminase from bovine spleen
Type IX, ammonium sulfate suspension, 150-200 units/mg protein
Sinonimo/i:
Adenosine aminohydrolase
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About This Item
Prodotti consigliati
Origine biologica
bovine spleen
Livello qualitativo
Tipo
Type IX
Stato
ammonium sulfate suspension
Attività specifica
150-200 units/mg protein
PM
32.5-33 kDa
N° accesso UniProt
Attività estranea
5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%
Temperatura di conservazione
2-8°C
Informazioni sul gene
cow ... ADA(280712)
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Descrizione generale
Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Applicazioni
Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Azioni biochim/fisiol
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.
Definizione di unità
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
Stato fisico
Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0
Risultati analitici
Protein determined by biuret.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articoli
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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