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MTOX1019

Sigma-Aldrich

MDR1 Knockout HepaRG™ Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.81

product name

MDR1 Knockout HepaRG™ Cells, 1 vial

Origine biologica

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

Livello qualitativo

200

Forma fisica

liquid

Temperatura di conservazione

−196°C

Informazioni sul gene

human ... ABCB1(5243)

Descrizione generale

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MDR1 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Applicazioni

See technical bulletin for detailed protocols

Caratteristiche e vantaggi

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

  • Sigma′s HepaRG MDR1 Knockout (KO) allows investigations of drug-transporter interactions involving MDR1 (P-glycoprotein) in the liver.

Zinc finger nucleases (ZFN) mediated Knock-out of MDR1 gene.

  • The frame-shift mutation of MDR1 gene was confirmed by fragment length analysis and DNA sequencing.
  • Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.

Qualità

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Note legali

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

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