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Merck
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Documenti fondamentali

MBD0043

Sigma-Aldrich

Vitamin K1-Hemin ready made solution

For bacterial culture

Sinonimo/i:

anaerobic media supplement

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About This Item

Codice UNSPSC:
41106212
NACRES:
NA.74

Livello qualitativo

Concentrazione

-100 ×

tecniche

FISH: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Hemin- Vitamin K1 readymade solution is used as a culture medium enrichment for fastidious anaerobic microorganisms.1,2 Hemin-Vitamin K1 is a key ingredient compound that is part of anaerobic media such as brucella broth, thioglycolate broth, reinforced clostridial medium, cooked meat broth, peptone yeast extract medium, fastidious anaerobe agar etc. Hemin helps to regulate the expression of numerous virulence factors and extracellular vesicle formations in Porphyromonas sp. 3,4. Vitamin K1 (phylloquinone) is produced by plants, it is used as a redox cofactor in plants and cyanobacteria. Vitamin K1 is converted into vitamin K2 in the gut by intestinal microbiota. Hemin and Vitamin K1have been shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore formers organisms.

Applicazioni

Hemin- Vitamin K1 readymade solution is suitable to use as a media supplement for anaerobic bacterial culture .

Caratteristiche e vantaggi

  • Enriches fastidious anaerobic microorganisms
  • Ready-made solution
  • Sterile filtered

Altre note

For each liter (L) of medium prepared, add 10-20 mL Hemin Vitamin K readymade solution after autoclaving.

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Flam. Liq. 3

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

120.2 °F

Punto d’infiammabilità (°C)

49 °C


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Teresa Olczak et al.
FEMS microbiology reviews, 29(1), 119-144 (2005-01-18)
Porphyromonas gingivalis is a Gram-negative anaerobic bacterium associated with the initiation and progression of adult periodontal disease. Iron is utilized by this pathogen in the form of heme and has been shown to play an essential role in its growth
J M Conly et al.
Progress in food & nutrition science, 16(4), 307-343 (1992-10-01)
Vitamin K is an essential cofactor necessary for the production of clotting factors II, VII, IX, and X in humans and has recently been found to be an essential factor for many other proteins in the body. There are two

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