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199303

Sigma-Aldrich

Resazurin sodium salt

certified by the BSC

Sinonimo/i:

7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

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About This Item

Formula empirica (notazione di Hill):
C12H6NNaO4
Numero CAS:
Peso molecolare:
251.17
Beilstein:
3794971
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

agenzia

certified by the BSC

Livello qualitativo

Stato

powder

Composizione

Dye content, 75%

Solubilità

1 M NH4OH: 1 mg/mL

λmax

380 nm (2nd)
598 nm

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1

InChI

1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
IVGPGQSSDLDOLH-UHFFFAOYSA-M

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Descrizione generale

Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.

Applicazioni

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:
  • the measurement of metabolic activity and the proliferation of living cells
  • the detection of bacterial reduction in milk
  • in viability assays as a component of culture media for the detection of viable bacteria
  • to determine the number of motile sperm in sheep semen
  • to assay lactate dehydrogenase
  • as an analytical reagent to quantify L-glutamate

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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Global Trade Item Number

SKUGTIN
199303-25G4061835570386
199303-1G4061838763112
199303-5G4061835570393

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