MAK558

Trypsin Activity Assay Kit

sufficient for 100 colorimetric tests

• Codice UNSPSC: 41116104

Proprietà

• impiego: sufficient for 100 colorimetric tests
• Livello qualitativo: 100
• Metodo di rivelazione: colorimetric
• Malattie correlate: gastrointestinal diseases
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Trypsin is a pancreatic serine protease, which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. Tryptic digestion of the protein of interest results in a highly specific cleavage and a limited number of peptide fragments. In this assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ= 405 nm. Trypsin activity can be accurately measured by the color intensity which is proportional to p-NA content. The kit detects 10–100 mU (p-NA unit) trypsin in various samples.

Caratteristiche e vantaggi

Highly sensitive, simple and high-throughput assay for measuring Trypsin concentration.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Compatibilità

Suitable for the detection of Trypsin in various biological samples such as cell and tissue extracts, serum, and plasma.

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H318,H335,H412
• Consigli di prudenza: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3