MAK524

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• applicazioni: food and beverages
• Metodo di rivelazione: colorimetric
• Malattie correlate: cancer
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Isocitrate (isocitric acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.

Caratteristiche e vantaggi

• Linear detection range: 20 to 5000 µM
• Compatible with high-throughput handling systems.

Compatibilità

The kit is suitable for isocitrate determination in food, beverages, and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).

Principio

The Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2