MAK521

Fumarase Assay Kit

sufficient for 100 colorimetric tests

• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• Metodo di rivelazione: colorimetric
• Malattie correlate: neurological disorders; neonatal diseases
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Fumarase (or fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.

Caratteristiche e vantaggi

• Linear detection range: 0.4 to 70 U/L
• Compatible with high-throughput handling systems.

Compatibilità

The kit is suitable for fumarase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Principio

The Fumarase Assay Kit is a non-radioactive, colorimetric assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H225,H290,H315,H319,H336
• Consigli di prudenza: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Central nervous system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3