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MAK117

Sigma-Aldrich

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Sinonimo/i:

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 200 colorimetric or fluorometric tests

applicazioni

cosmetics
environmental
food and beverages

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

cancer

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Applicazioni

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principio

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Codice della classe di stoccaggio

10 - Combustible liquids


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Background: Impaired capillary growth (angiogenesis) in skeletal muscle and adipose tissue contributes to the development of metabolic disorders in obese males. This association remains unexplored in females, despite mounting evidence that endothelial cells have sex-specific transcriptional profiles. Therefore, herein we
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Nicolae S.
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Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
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Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether

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