FG0100
Kit e reagenti per trigliceridi e glicerolo libero
1 kit sufficient for 1000 reactions
Sinonimo/i:
Triglyceride and Free Glycerol Kits and Reagents
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.26
Prodotti consigliati
impiego
kit sufficient for 1000 reactions
Metodo di rivelazione
colorimetric
Temperatura di conservazione
2-8°C
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Descrizione generale
Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
Applicazioni
I kit dei trigliceridi e del glicerolo libero servono alla determinazione quantitativa di glicerolo, trigliceridi totali o trigliceridi liberi.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.
Compatibilità
Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
Linkage
Oltre ai kit, i singoli reagenti e lo standard del glicerolo sono disponibili separatamente quando è necessario eseguire un minor numero di reazioni.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
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