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MAK178

Sigma-Aldrich

Enolase Activity Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinonimo/i:

Enolase Detection Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

immunological diseases; cardiovascular diseases

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)

Categorie correlate

Descrizione generale

Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.

Applicazioni

Enolase Activity Assay Kit has been used to measure enolase activity.

Compatibilità

Suitable for the detection of enolase activity in biological samples.

Principio

The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.

Solo come componenti del kit

N° Catalogo
Descrizione
  • Enolase Assay Buffer
  • Peroxidase Substrate, in DMSO
  • Enolase Substrate Mix
  • Enolase Converter
  • Enolase Developer
  • Enolase Positive Control
  • Hydrogen Peroxide Standard, 0.88 M

Indicazioni di pericolo

H412

Consigli di prudenza

P273 - P501

Classi di pericolo

Aquatic Chronic 3

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH Annex XIV (Authorisation List)

Certificati d'analisi (COA)

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Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism.
Bontemps-Gallo S, et al.
Frontiers in Microbiology, 9, 2036-2036 (2018)
WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma.
Chen S, et al.
Cell Death & Disease, 9(3), 347-347 (2018)
Jiayu Zhou et al.
Cell death & disease, 10(12), 885-885 (2019-11-27)
Lung adenocarcinoma (LUAD) has long been one of the predominant reasons for the global cancer-linked mortality. The tumor progression is shown by several studies to be promoted by increased glycolysis. Enolase 1 (ENO1), as a glycolysis enzyme, performs pivotal role
Sébastien Bontemps-Gallo et al.
Frontiers in microbiology, 9, 2036-2036 (2018-09-21)
The post-translational modification of proteins has been shown to be extremely important in prokaryotes. Using a highly sensitive mass spectrometry-based proteomics approach, we have characterized the acetylome of B. burgdorferi. As previously reported for other bacteria, a relatively low number
Xing Huang et al.
Autophagy, 15(7), 1258-1279 (2019-02-23)
Notwithstanding the numerous drugs available for liver cancer, emerging evidence suggests that chemotherapeutic resistance is a significant issue. HGF and its receptor MET play critical roles in liver carcinogenesis and metastasis, mainly dependent on the activity of receptor tyrosine kinase.

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