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Merck
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MAK165

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

Sinonimo/i:

Hydrogen Peroxide Quantification Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 500 fluorometric tests (red fluorescence)

Metodo di rivelazione

fluorometric

Malattie correlate

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

Temperatura di conservazione

−20°C

Descrizione generale

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principio

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Oral - Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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