Passa al contenuto
Merck
Tutte le immagini(3)

Documenti fondamentali

MAK095

Sigma-Aldrich

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

endocrinological disorders, diabetes; neurological disorders

Temperatura di conservazione

−20°C

Descrizione generale

Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.

Applicazioni

Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.

Compatibilità

The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principio

Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup


Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Influence of ketamine on amino acid neurotransmitters secretion by nerve cells in vitro.
Shi M, et al.
Bangladesh Journal of Pharmacology, 11(S1), 55-60 (2016)
The Impact of Inflammation on Pancreatic β-Cell Metabolism, Function and Failure in T1DM and T2DM: Commonalities and Differences.
Newsholme P, et al.
Type 1 Diabetes, 125-165 (2013)
Essentials of medical biochemistry: with clinical cases.
Ha C E and Bhagavan N V
ESSENTIALS OF MEDICINAL CHEMISTRY, 2011 null
Role of the Malate-Aspartate Shuttle on the Metabolic Response to Myocardial Ischemia
Ming Lu, et al.
Journal of Theoretical Biology, 254(2), 466-475 (2008)
Philip H Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(35), E7226-E7235 (2017-08-16)
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC)

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.