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Key Documents

MAK061

Sigma-Aldrich

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

Metodo di rivelazione

colorimetric

Temperatura di conservazione

−20°C

Descrizione generale

The new Isocitrate Assay Kit, MAK524, is now available! Isocitrate is a TCA cycle intermediate that is formed from citrate by the activity of the enzyme aconitase. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. In plants and lower organisms, isocitrate is the branch point from which the glyoxylate shunt operates. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. The Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples.

Compatibilità

Suitable for quantifying isocitrate in a variety of samples including tissue, cells, food and beverages

Principio

Isocitrate concentration is determined by a coupled enzyme assay, which results in a colorometric (450 nm) product, proportional to the isocitrate present. Typical sensitivities of detection for this kit are 1-20 nmoles (0.2 - 5 μg) of isocitrate.

Applicazioni

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazardCorrosion

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


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Articoli

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

Protocolli

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

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