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Documenti fondamentali

M5524

Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

Sinonimo/i:

MS Basal Salts, MS0 Basal Salts, MSO Basal Salts

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.72

Stato

powder

Livello qualitativo

100

tecniche

cell culture | plant: suitable

applicazioni

agriculture

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Descrizione generale

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Applicazioni

Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells. It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.

Formula variabile

With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Quantità

Formulated to contain 4.3 grams of powder per liter of medium.

Nota sulla preparazione

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Altre note

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

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08168

Timestrip Plus™ 8 °C

Pittogrammi

Flame over circleExclamation mark
GHS03,GHS07

Avvertenze

Warning

Indicazioni di pericolo

H272,H319

Classi di pericolo

Eye Irrit. 2 - Ox. Sol. 3

Codice della classe di stoccaggio

5.1B - Oxidizing hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLCH8257
SLCF5871
SLCF0742
SLCC6355
SLCC5900

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Articoli

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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