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M5257

Sigma-Aldrich

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Sinonimo/i:

L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme

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About This Item

Numero CAS:
9028-47-1
Classificazione EC (Enzyme Commission):
1.1.1.40 (BRENDA, IUBMB)
Numero CE:
232-834-8
Numero MDL:
MFCD00131568
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

chicken liver

Forma fisica

ammonium sulfate suspension

Attività specifica

10-30 units/mg protein (modified Warburg-Christian)

Temperatura di conservazione

2-8°C

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Descrizione generale

Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.

Applicazioni

Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.

Azioni biochim/fisiol

Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.

Definizione di unità

One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.

Stato fisico

Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Phosphoenolpyruvate carboxylase microbial ≥5.0 unit/mg solid

Sigma-Aldrich

C1744

Phosphoenolpyruvate carboxylase microbial

Glutamic-Oxalacetic Transaminase from porcine heart Type I, ammonium sulfate suspension, 200-500 units/mg protein

Sigma-Aldrich

G2751

Glutamic-Oxalacetic Transaminase from porcine heart

A R Ashton
Archives of biochemistry and biophysics, 345(2), 251-258 (1997-10-06)
NADP-malic enzyme (NADP-ME, EC 1.1.1.40) was purified to near-homogeneity from leaves of the C4 dicot Flaveria bidentis and shown to possess intrinsic NAD-dependent malic enzyme activity. The NAD-dependent activity is optimal at pH 7.5 and in the presence of Mn2+.
Synthesis and Degradation of Malic Enzyme in Chick Liver.
Silpananta, P
The Journal of Biological Chemistry, 264, 5754-5761 (1971)
K N Rao et al.
Gastroenterology, 113(1), 238-248 (1997-07-01)
We showed previously that the peroxisome proliferators di(2-ethylhexyl)phthalate (DEHP), clofibrate, and 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyl)acetamide (BR931) alter hepatic sex steroid metabolism and receptor expression during induction of hepatic hyperplasia and hepatocellular carcinoma (HCC) in rats. The aim of this study was
Establishment and biological characterization of fibroblast cell line from the Langshan chicken
Guan, W., et al.
Cell Proliferation, 42, 157-163 (2010)
Dietary manganese requirement of juvenile yellow catfish Pelteobagrus fulvidraco, and effects on whole body mineral composition and hepatic intermediary metabolism
Tan, X., et al.
Aquaculture (Amsterdam, Netherlands), 326, 68-73 (2012)
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