L7261
Lipopolysaccharides from Salmonella enterica serotype typhimurium
purified by trichloroacetic acid extraction
Sinonimo/i:
LPS
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About This Item
Prodotti consigliati
Origine biologica
Salmonella enterica (Serotype typhimurium)
Livello qualitativo
Stato
lyophilized powder
Purificato mediante
trichloroacetic acid extraction
Impurezze
1-10% Protein (Lowry)
Colore
white to faint yellow
Solubilità
water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
This product is TCA extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS serotype has been used to induce NOS in rats and guinea pigs.
Applicazioni
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Azioni biochim/fisiol
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Nota sulla preparazione
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Altre note
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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