L2650

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

• Sinonimo/i: SBA, Soybean agglutinin
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: Glycine max (soybean)
• Coniugato: peroxidase conjugate
• Saggio: 85.00-100.00% (Warburg-Christian)
• Forma fisica: lyophilized powder
• Potenza: <80 μg per mL agglutination activity
• peroxidase activity: 25-100 units/mg protein
• Composizione: Protein, ~95% modified Warburg-Christian
• tecniche: western blot: suitable
• N° accesso UniProt: P05046
• Temperatura di conservazione: −20°C
• Informazioni sul gene: soybean ... LE1(100818710)

Descrizione

Applicazioni

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Azioni biochim/fisiol

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Definizione di unità

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Stato fisico

Contains sodium citrate

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Risultati analitici

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)