I8135

IgM from bovine serum

reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution

• Sinonimo/i: Bovine IgM
• Numero MDL: MFCD00163154
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Coniugato: unconjugated
• Livello qualitativo: 300
• Grado: reagent grade
• Saggio: ≥95% (SDS-PAGE or HPLC)
• Forma fisica: buffered aqueous solution
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from bovine serum is purified from normal bovine serum by precipitation and gel filtration techniques.

IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.

Applicazioni

IgM from bovine serum has been used for screening the lipopolysaccharide levels in Limulus amoebocyte lysate (LAL) assay. IgM has been used in flow cytometry assay for detecting FcγRIV expression and immunoglobulin binding.

Purified bovine IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.

Azioni biochim/fisiol

IgM plays a key role in engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Stato fisico

Solution in 0.05 M Tris-HCl, 0.15 M sodium chloride and 0.01 M glycine, pH 7.4, containing 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable