H5505
Histone from calf thymus
Type III-S
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About This Item
Prodotti consigliati
Origine biologica
bovine (calf) thymus
Livello qualitativo
Tipo
Type III-S
Forma fisica
powder
Concentrazione
≥60% protein (biuret)
tecniche
activity assay: suitable
Solubilità
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
Temperatura di conservazione
2-8°C
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Applicazioni
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
- in Cdk5 activity assay
- in histone H1 kinase assay
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells
Azioni biochim/fisiol
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.
Caratteristiche e vantaggi
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Altre note
Characterized as mainly subgroup f1 by SDS-PAGE
Nota sulla preparazione
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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