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H5000

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

Sinonimo/i:

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast

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About This Item

Numero CAS:
9001-51-8
Classificazione EC (Enzyme Commission):
2.7.1.1 (BRENDA, IUBMB)
Numero CE:
232-611-5
Numero MDL:
MFCD00131321
Codice UNSPSC:
12352204
eCl@ss:
32160410

Tipo

Type III

Forma fisica

lyophilized powder

Attività specifica

≥25 units/mg protein (biuret)

PM

dimer 110 kDa

N° accesso UniProt

P04806
P04807

Attività estranea

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

Temperatura di conservazione

−20°C

Informazioni sul gene

bakers yeast ... HXK1(850614) , HXK2(852639)

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Azioni biochim/fisiol

Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM

Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

Definizione di unità

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

Stato fisico

Lyophilized powder containing approx. 15% sodium citrate

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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The Journal of cell biology, 203(4), 563-574 (2014-01-05)
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Protocolli

Enzymatic Assay of Hexokinase

Enzymatic Assay of Hexokinase

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