H4290

Hydroxyphenyl fluorescein

5 mM in DMF

• Sinonimo/i: 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, HPF
• Formula empirica (notazione di Hill): C₂₆H₁₆O₆
• Peso molecolare: 424.40
• Numero CE: 200-679-5
• Codice UNSPSC: 12352108
• NACRES: NA.32

Proprietà

• Livello qualitativo: 200
• Concentrazione: 5 mM in DMF
• Fluorescenza: λ_ex 490 nm; λ_em 515 nm
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Hydroxyphenyl fluorescein solution, also known as HPF is a fluorescent probe, which upon oxidation by the hydroxyl radical (HO) returns a brilliant green fluorescence (HPF-ox). Increased HPF signals in antibiotic-exposed bacterial cells are explained by fluorescence associated with increased cell size, not by ROS levels. In cells incubated with the HPF probe and exposed to ampicillin and norfloxacin with HPF fluorescence correlated linearly.

Applicazioni

Fluorescent reagent for the detection of highly reactive oxygen species (hROS). Fluorescence intensity increases after the immediate reaction with hROS such as hydroxyl radical and peroxynitrite. Since HPF does not react with nitric oxide, superoxide and hydrogen peroxide, it can differentially detect peroxynitrite. HPF is resistant to light-induced autoxidation.

Hydroxyphenyl fluorescein solution is used to detect highly reactive oxygen species (hROS). It is a suitable probe to detect cellular death caused by bacterial antibiotics.

Caratteristiche e vantaggi

The following are the benefits of Hydroxyphenyl fluorescein solution:

• HPF is resistant to light-induced autooxidation.
• Hypochlorite(-OCl) does not react with HPF.
• HPF does not react with nitric oxide, superoxide, and hydrogen peroxide. 
• HPF has low reactivity with HOCl.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H226,H312 + H332,H319,H360D
• Consigli di prudenza: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 135.5 °F - (Dimethylformamide)
• Punto d’infiammabilità (°C): 57.5 °C - (Dimethylformamide)
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter