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Key Documents

H17001

Sigma-Aldrich

Wnt3A, human

recombinant, expressed in HEK 293 cells human

Sinonimo/i:

Wnt, Wnt-3A

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.77

Ricombinante

expressed in HEK 293 cells human

Saggio

>90% (SDS-PAGE)

Forma fisica

powder

Potenza

≤20 ng/mL EC50

PM

38-42 kDa

Impurezze

<1 EU/μg endotoxin

Temperatura di conservazione

−20°C

Descrizione generale

WNT3A (Wnt family member 3A) gene is mapped to human chromosome 1q42.13. Wnt3A gene is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins.

Azioni biochim/fisiol

WNT3A (Wnt family member 3A) functions mainly to induce canonical Wnt signaling pathway. Wnt signaling pathway is essential for gastrulation, organogenesis, tissue development, homeostasis and regeneration. Congenital defects, oncogenic transformation and many more disorders results due to abnormal Wnt signaling pathway. During the differentiation of human neuronal progenitor cells, WNT3A (Wnt family member 3A) activates calmodulin-dependent kinase II and increases free calcium in the cytosol via Wnt/Ca2+ signaling pathway. WNT3A is an oncogene and is upregulated in many types of cancer. It serves as a prognostic marker in diagnosing hepatocellular carcinoma. It also promotes metastasis of malignant tumors, including gastric cancer, colorectal cancer, non-small cell lung cancer and breast cancer.

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 1X PBS pH 7.4 + 500mM NaCl + 1% CHAPS.

Ricostituzione

It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1X PBS containing 0.1% endotoxin-free HSA.

Stoccaggio e stabilità

Upon reconstitution, the cytokine can be stored at 4°C for short term or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.

Risultati analitici

The activity was determined by dose dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells and stabilization of beta catenin in mouse L929 cells.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Expression of Wnt3a in hepatocellular carcinoma and its effects on cell cycle and metastasis
Lu C, et al.
International Journal of Oncology, 51(4), 1135-1145 (2017)
Ying Xu et al.
Frontiers in bioengineering and biotechnology, 8, 946-946 (2020-08-28)
Mesenchymal stromal cell senescence and apoptosis have been identified as critical molecular hallmarks in aging. In this study, we used stromal cell sheet culture as an in vitro model to study the progressive changes of cellular senescence, apoptosis and underlying
The Wnt inhibitor LGK-974 enhances radiosensitivity of HepG2 cells by modulating Nrf2 signaling
Tian D, et al.
International Journal of Oncology, 51(2), 545-554 (2017)
The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells
Huggins IJ, et al.
Nature Communications, 8(1), 1034-1034 (2017)
Cross-talk of WNT and FGF signaling pathways at GSK3beta to regulate beta-catenin and SNAIL signaling cascades.
Katoh M and Katoh M
Cancer Biology & Therapy, 5(9), 1059-1064 (2006)

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