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H1506

Sigma-Aldrich

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Sinonimo/i:

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

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About This Item

Numero CAS:
9028-56-2
Classificazione EC (Enzyme Commission):
1.1.1.50 (BRENDA, IUBMB)
Numero CE:
232-839-5
Numero MDL:
MFCD00130298
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bacterial (Pseudomonas testosteroni)

Forma fisica

lyophilized solid

Attività specifica

≥15 units/mg protein

Purificato mediante

chromatography

Composizione

Protein, ≥25%

Attività estranea

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

Temperatura di conservazione

−20°C

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Applicazioni

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Azioni biochim/fisiol

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Definizione di unità

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Stato fisico

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sigma-Aldrich

221368

Sodium pyrophosphate decahydrate

β-Dinucleotide nicotinamide adenina Grade I, free acid

Roche

NAD100-RO

β-Dinucleotide nicotinamide adenina

Perossidasi Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

Sigma-Aldrich

P8375

Perossidasi

Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Expression and characterization of recombinant type 2 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha17beta-HSD activity and cellular distribution
Lin HK, et al.
Molecular Endocrinology, 11(13), 1971-1984 (1997)
Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
Brandon Bartling et al.
The Analyst, 134(5), 973-979 (2009-04-22)
An electrochemical sensor, based on thick-film screen-printed Ir/C working and counter electrodes, was developed for the detection of total bile acid concentration in a physiological fluid for potential patient management in patients with liver disease. Current electrochemical methods of detecting
Frank Hoffmann et al.
Journal of biotechnology, 129(1), 131-139 (2007-01-30)
3alpha-Hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily and catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid. The enzyme also mediates the carbonyl reduction of
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Protocolli

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)

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