GERPN3000
ECL™ Direct Nucleic Acid
Cytiva RPN3000, pack of 1 ea
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About This Item
Codice UNSPSC:
41105300
NACRES:
NA.31
Prodotti consigliati
Confezionamento
pack of 1 ea
Produttore/marchio commerciale
Cytiva RPN3000
Temperatura di conservazione
2-8°C
Descrizione generale
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Applicazioni
ECL™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.
Caratteristiche e vantaggi
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very Low backgrounds.
Stoccaggio e stabilità
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Note legali
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
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Certificati d'analisi (COA)
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Articoli
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
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