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Key Documents

GE29-0486-84

HiTrap® Desalting

Cytiva 29-0486-84, pack of 5 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

Confezionamento

pack of 5 mL

Produttore/marchio commerciale

Cytiva 29-0486-84

Disponibilità

not available in North America

Condizioni di stoccaggio

dry media

Parametri

42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

cross-linked dextran

Dimensione particelle

>20 μm (dry)

cleaning

2-13

working range

2-13

Compatibilità

suitable for bioprocess medium

Descrizione generale

HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Applicazioni

Gel filtration has successfully been employed for size based separations of macromolecules since the late 1950s. Gel filtration, or size exclusion chromatography, may be performed in three principally different modes, depending on the size differences of the solutes to be separated (i) group separation (ii) fractionation (iii) determination of molecular mass (distributions) When the size difference is large, i.e. a factor of more than 10, we talk about group separation (e.g. desalting, buffer exchange) and when the size difference is small, i.e. a factor of 2 to 5, we talk about fractionation. The third mode is analytical gel filtration, e.g. determination of molecular mass distributions, where an array of molecular masses is to be separated. These different modes put different requirements on the equipment and media used and will yield widely different productivity in terms of information or material processed.

Caratteristiche e vantaggi

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex G-25 and the optimized packing technology.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva

Prodotti correlati

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Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

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Protocolli

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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