GE28-9924-73
HiScreen™ Capto™ Butyl
Cytiva 28-9924-73, pack of 1 column
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About This Item
Codice UNSPSC:
23151817
NACRES:
NA.56
ligand
butyl
Confezionamento
pack of 1 column
Produttore/marchio commerciale
Cytiva 28-9924-73
Dimensioni del letto
7.7 mm × 100 mm
Diam. int. colonna
7.7 mm
Matrice
highly cross-linked agarose
Dimens. media delle particelle
75 μm (d50v)
cleaning
3-14
working range
3-13
Capacità
27 mg binding capacity(27 mg BSA /ml (at QB10%))
Compatibilità
suitable for bioprocess medium
Descrizione generale
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
Applicazioni
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Caratteristiche e vantaggi
- Prepacked with Capto™ Butyl
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
Stoccaggio e stabilità
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Note legali
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
Prodotti correlati
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Descrizione
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Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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