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GE28-9187-78

MBPTrap HP

Cytiva 28-9187-78, pack of 5 × 1 mL

Sinonimo/i:

MBPTrap HP

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

dextrin

Confezionamento

pack of 5 × 1 mL

Produttore/marchio commerciale

Cytiva 28-9187-78

Condizioni di stoccaggio

(20% Ehtanol)

Parametri

42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-13

Intervallo di esercizio

>7

Capacità

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

Temperatura di conservazione

2-8°C

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Descrizione generale

MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Caratteristiche e vantaggi

  • HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
  • Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
  • Physiological conditions and mild elution preserve the activity of the target protein.
  • Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

MBPTrap is a trademark of Cytiva

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Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Certificati d'analisi (COA)

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Articoli

This page shows troubleshooting of methods for purification of MBP-tagged recombinant proteins using GE Healthcare products.

This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.

Protocolli

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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