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Key Documents

GE28-9355-97

Dextrin Sepharose™ High Performance

Cytiva 28-9355-97, pack of 25 mL

Sinonimo/i:

Dextrin Sepharose, High Performance Dextrin Resin

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

dextrin

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 28-9355-97

Condizioni di stoccaggio

(20% Ehtanol)

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning in place

2-13

working range

>7

Capacità

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

Temperatura di conservazione

2-8°C

Descrizione generale

Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Applicazioni

Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Caratteristiche e vantaggi

  • Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
  • The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
  • Physiological conditions and mild elution preserve target Protein activity
  • Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Proteasi TEV

Sigma-Aldrich

T4455

Proteasi TEV

Articoli

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.

Protocolli

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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