GE17-5157-01
HiTrap® SP Fast Flow
Cytiva 17-5157-01, pack of 5 × 5 mL
Sinonimo/i:
HiTrap SP
About This Item
Prodotti consigliati
ligand
sulphopropyl
Descrizione
Ion Exchanger Type (value)
Confezionamento
pack of 5 × 5 mL
Produttore/marchio commerciale
Cytiva 17-5157-01
Parametri
flow rate
42 psi
Dimensioni del letto
16 mm × 25 mm
Volume del letto
5 mL
Diam. int. colonna
16 mm
Matrice
6% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning
3-14
working range
4-13
Compatibilità
suitable for bioprocess medium
Descrizione generale
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
Caratteristiche e vantaggi
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Stoccaggio e stabilità
Risultati analitici
Altre note
Note legali
Prodotti correlati
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocolli
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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