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Key Documents

GE17-5157-01

HiTrap® SP Fast Flow

Cytiva 17-5157-01, pack of 5 × 5 mL

Sinonimo/i:

HiTrap SP

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

sulphopropyl

Descrizione

Ion Exchanger Type (value)

Confezionamento

pack of 5 × 5 mL

Produttore/marchio commerciale

Cytiva 17-5157-01

Parametri

flow rate
42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

6% cross-linked agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning

3-14

working range

4-13

Compatibilità

suitable for bioprocess medium

Descrizione generale

HiTrap® SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Caratteristiche e vantaggi

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Prodotti correlati

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Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Vedi tutto

Protocolli

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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