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Key Documents

GE17-5068-01

Sigma-Aldrich

SOURCE 15RPC ST 4.6/100

pkg of 1 column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

ligand

matrix surface

Materiali

polypropylene column

Caratteristiche

autoclavable 20 min at 121°C

Confezionamento

pkg of 1 column

Parametri

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

tecniche

HPLC: suitable

Dimensioni del letto

4.6 mm × 100 mm

Volume del letto

1.7 mL

Diam. int. colonna

4.6 mm

Matrice

polymeric
polystyrene/divinylbenzene

Gruppo funzionale matrice

polystyrene-divinylbenzene phase

Dimens. media delle particelle

15 μm

cleaning

1-14

working range

1-12

Capacità

~10 mg binding capacity

Compatibilità

suitable for bioprocess medium

Tecnica di separazione

reversed phase

Descrizione generale

SOURCE 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Applicazioni

SOURCE 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE 15RPC offers properties superior to those of other polymeric matrices.

Caratteristiche e vantaggi

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

This page discusses column packing and preparation techniques for reverse phase chromatography

Protocolli

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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