GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Sinonimo/i:
Resource Q Column
About This Item
Prodotti consigliati
ligand
quaternary ammonium
Descrizione
Ion Exchanger Type (value)
Confezionamento
pack of 6 mL
Produttore/marchio commerciale
Cytiva 17-1179-01
Parametri
87 psi
Dimensioni del letto
16 mm × 30 mm
Volume del letto
6 mL
Matrice
polystyrene/divinylbenzene
Dimens. media delle particelle
15 μm
Dimensione particelle
15-15 μm
cleaning
1-14
working range
2-12
Compatibilità
suitable for bioprocess medium
Categorie correlate
Descrizione generale
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Applicazioni
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Caratteristiche e vantaggi
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Stoccaggio e stabilità
Risultati analitici
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Protocolli
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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