GE17-0709-10
DEAE Sepharose™ Fast Flow
Cytiva 17-0709-10, pack of 25 mL
Sinonimo/i:
Anion exchange purification
About This Item
Prodotti consigliati
ligand
diethylaminoethyl
Descrizione
Ion Exchanger Type (value)
Confezionamento
pack of 25 mL
Produttore/marchio commerciale
Cytiva 17-0709-10
Matrice
6% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning in place
2-14
working range
2-12
Compatibilità
suitable for bioprocess medium
Descrizione generale
Applicazioni
As member of the BioProcess media range, DEAE Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Caratteristiche e vantaggi
- Well-proven weak anion exchanger developed for industrial downstream processes
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.
Stoccaggio e stabilità
Risultati analitici
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocolli
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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