G2791

Anti-Grb-2 antibody, Mouse monoclonal

clone GRB-232, purified from hybridoma cell culture

• Numero MDL: MFCD00802742
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: GRB-232, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 24 kDa
• Reattività contro le specie: rat, mouse, human
• Concentrazione: ~2 mg/mL
• tecniche: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using a whole extract of cultured human acute T-cell leukemia Jurkat cells
• Isotipo: IgG3
• N° accesso UniProt: P62993
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GRB2(2885) ; mouse ... Grb2(14784) ; rat ... Grb2(81504)

Descrizione

Descrizione generale

Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer.
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).

Immunogeno

synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2.

Applicazioni

Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota sulla preparazione

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.