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G1166

Sigma-Aldrich

Monoclonal Anti-Glutamic Acid Decarboxylase 65 antibody produced in mouse

clone GAD-6, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Anti-GAD 65

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About This Item

Numero MDL:
MFCD03455043
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

GAD-6, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mammals (and some lower vertebrates)

tecniche

immunohistochemistry: 1:100-1:500
western blot: 1:1,000-1:5,000

Isotipo

IgG2a

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... GAD2(2572)
mouse ... Gad2(14417)

Specificità

Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain, GAD 65. Can be used for immunochemical localization of GABA secreting neurons.

Immunogeno

purified rat brain GAD 65.

Stato fisico

Solution in 0.01 M phosphate buffered saline, containing 0.04% sodium azide.

Nota sulla preparazione

Purified using Protein A chromatography.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Gregory J Brewer et al.
Frontiers in neural circuits, 7, 165-165 (2013-10-25)
The mammalian hippocampus functions to encode and retrieve memories by transiently changing synaptic strengths, yet encoding in individual subregions for transmission between regions remains poorly understood. Toward the goal of better understanding the coding in the trisynaptic pathway from the
M Esclapez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 14(3 Pt 2), 1834-1855 (1994-03-01)
Two isoforms of glutamic acid decarboxylase (GAD67 and GAD65) and their mRNAs were localized in the rat brain by immunohistochemistry and nonradioactive in situ hybridization methods with digoxigenin-labeled cRNA probes. In most brain regions, both GAD isoforms were present in
Seung T Lim et al.
The Journal of comparative neurology, 507(2), 1228-1244 (2008-01-09)
Nectins are cell adhesion molecules that, together with the intracellular binding partner afadin, mediate adhesion and signaling at a variety of intercellular junctions. In this work we studied the distribution of nectin-1 and afadin during hippocampal synapse formation using cultured
Ran-Sook Woo et al.
Neuron, 54(4), 599-610 (2007-05-25)
Neuregulin-1 (NRG1), a regulator of neural development, has been shown to regulate neurotransmission at excitatory synapses. Although ErbB4, a key NRG1 receptor, is expressed in glutamic acid decarboxylase (GAD)-positive neurons, little is known about its role in GABAergic transmission. We
Stathis S Leondopulos et al.
Journal of neural engineering, 9(2), 026015-026015 (2012-03-01)
Slow wave oscillations in the brain are essential for coordinated network activity but have not been shown to self-organize in vitro. Here, the development of dissociated hippocampal neurons into an active network with oscillations on multi-electrode arrays was evaluated in
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