G0413

β(1→4)-Galactosidase, positionally specific from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• CAS Number: 9031-11-2
• Numero CE: 3.2.1.23 (BRENDA, IUBMB)
• Numero MDL: MFCD01092684
• Codice UNSPSC: 12352204
• NACRES: NA.32

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 100
• Stato: buffered aqueous solution
• Attività specifica: ≥6 units/mg protein
• Confezionamento: vial of 0.06 unit
• N° accesso UniProt: P16278
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: human ... GLB1(2720)

Descrizione

Descrizione generale

β-Galactosidase is present in bacteria, fungi, yeast and animal organs. It is also found in fruits, such as apples, almonds and apricots. β-Galactosidase is a tetramer and is made up of four polypeptide chains consisting of amino acids that assemble to form five structural domains. The domains are jelly roll barrel, a central domain that serves as an active site and the remaining domains are composed of β-sandwich and fibronectin.

Applicazioni

β(1→4)-Galactosidase, positionally specific from Streptococcus pneumonia has been used:

• as a position-specific enzyme to study its effects in the terminal galactosylation with protective efficacy of glycosphingolipid (GSPL) in mice.
• for the digestion of radioactive oligosaccharides.
• as a position-specific enzymeto study its effects on the virulence profile of avirulent Leishmania donovani clone (A-LD).

Azioni biochim/fisiol

β-Galactosidase plays a role in hydrolyzing the D-galactosyl moieties in oligosaccharides, polymers and secondary metabolites. It is widely applicable in the dairy industry to remove lactose from milk and dairy products for the benefit of lactose-intolerant individuals. β-Galactosidase is also applicable in the food industry to improve the sweetness, flavor and solubility.

Definizione di unità

One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-galactopyranoside per min at pH 5.0 at 37 °C.

Stato fisico

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable