F7250

Fluorescein isothiocyanate isomer I

≥90% purity (HPLC), powder

• Sinonimo/i: FITC, Fluorescein 5-isothiocyanate
• Formula empirica (notazione di Hill): C₂₁H₁₁NO₅S
• Numero CAS: 3326-32-7
• Peso molecolare: 389.38
• Beilstein: 1407295
• Numero CE: 222-042-0
• Numero MDL: MFCD00005063
• Codice UNSPSC: 12171500
• ID PubChem: 24894933
• NACRES: NA.47

Proprietà

• product name: Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
• Livello qualitativo: 200
• Saggio: ≥90% (HPLC)
• Forma fisica: powder
• tecniche: titration: suitable
• Colore: orange to dark orange
• Punto di fusione: >360 °C (lit.)
• Solubilità: acetone: 1 mg/mL
• Fluorescenza: λ_ex 492 nm; λ_em 518 nm (green)
• Compatibilità: suitable for protein labeling
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H; MHMNJMPURVTYEJ-UHFFFAOYSA-N

Descrizione

Descrizione generale

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Applicazioni

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317,H334
• Consigli di prudenza: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Faceshields, Gloves