F6258
Anti-Rat IgG (whole molecule)–FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinonimo/i:
Goat Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
goat
Coniugato
FITC conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
tecniche
direct immunofluorescence: 1:32
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Rat IgG (whole molecule)-FITC antibody is specific for all rat IgG subclasses. Goat anti-rat IgG is purified by affinity isolation and then conjugated to FITC.
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-rat IgG (whole molecule)-FITC antibody can be used as a secondary antibody in indirect immunofluorescence. Goat anti- rat IgG (whole molecule)-FITC antibody reacts specifically with all rat immunoglobulins.
Immunogeno
Purified rat IgG
Purified rat IgG.
Applicazioni
Anti-Rat IgG (whole molecule)−FITC antibody has been used in immunofluorescence measurement of antibody-treated cells and staining of monolayers.
Anti-Rat IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of rat spleen cells. For immunofluorescence of rat cartilage tissue sections, a concentration of 5-10 μg/ml was used. The range of dilutions of anti-rat IgG used for immunofluorescence is between 1:10 and 1:250 in various studies.. The antibody was also used in flow cytometry applications.
Anti-rat IgG (whole molecule)-FITC antibody (diluted 1:20 in PBS) can be used as a secondary antibody in immunolabelling. It can also be used in flow cytometry.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Sim K Singhrao et al.
Microscopy research and technique, 72(7), 501-506 (2009-03-21)
Immunofluorescence labeling on postembedded tissue in resin is a formidable task. Although resin components and stabilizers are a source of additional strong native fluorescence that overlaps with absorption and emission spectra of commonly used green fluorophores, the unfixed tissue is
T A Yednock et al.
The Journal of cell biology, 104(3), 725-731 (1987-03-01)
Blood-borne lymphocytes extravasate in large numbers within peripheral lymph nodes (PN) and other secondary lymphoid organs. It has been proposed that the initiation of extravasation is based upon a family of cell adhesion molecules (homing receptors) that mediate lymphocyte attachment
The Laboratory Rat (2005)
Aged HCT-8 cell monolayers support Cryptosporidium parvum infection
Sifuentes L Y, et al.
Applied and Environmental Microbiology, 73(23), 7548-7551 (2007)
Molecular Genetics of Immunoglobulin (1987)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.