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Key Documents

F6136

Sigma-Aldrich

Anti-Horse Spleen Ferritin antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Sinonimo/i:

Ferritin Antibody

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

lyophilized powder

Reattività contro le specie

horse

tecniche

immunoelectrophoresis: suitable
immunoelectrophoresis: suitable
indirect ELISA: 1:10,000

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

horse ... Ftl(100051593)

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Descrizione generale

Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains.
Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences.
.

Specificità

The antibody specifically recognizes ferritin from horse spleen.

Immunogeno

Purified horse spleen ferritin

Applicazioni

Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis.
Horse Spleen Ferritin antibody has been used:
  • immunofluorescence
  • in western blotting
  • in immunohistochemistry

Azioni biochim/fisiol

Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase.

Stato fisico

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Ricostituzione

Reconstitute with 0.135 M sodium chloride.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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Transferrin ensures survival of ovarian carcinoma cells when apoptosis is induced by TNF$\alpha$, FasL, TRAIL, or Myc
Fassl S, et al.
Oncogene, 22(51), 8343-8343 (2003)
Jan A Gorter et al.
Epilepsia, 46(9), 1371-1379 (2005-09-09)
Iron accumulation in the brain has been associated with neurodegenerative disorders, including epilepsy. In our previous SAGE study, we showed that ferritin, an iron-storage protein, was one of the genes (Ferritin-H) that showed overexpression before the chronic epileptic phase. In
New functions for an iron storage protein: the role of ferritin in immunity and autoimmunity
Recalcati S, et al.
Journal of Autoimmunity, 30(1-2), 84-89 (2008)
Microglial pathology in Down syndrome
Xue Q S and Streit W J
Acta Neuropathologica, 122(4), 455-455 (2011)
Structure and physiological functions of ferritin
Granick, S
Physiological Reviews, 31(4), 489-511 (1951)

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