F3385
Fetuin from fetal bovine serum
lyophilized powder, BioReagent, suitable for cell culture
Sinonimo/i:
Fetuin from fetal calf serum
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About This Item
Prodotti consigliati
Nome Commerciale
BioReagent
Stato
lyophilized powder
PM
48.4 kDa
tecniche
cell culture | mammalian: suitable
Impurezze
≤0.3% free N-acetylneuraminic acid
Solubilità
H2O: 1 mg/mL
Temperatura di conservazione
2-8°C
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Descrizione generale
Fetuin from fetal bovine serum is a plasma glycoprotein. It is a 64 kDa glycoprotein, which is produced by the liver and adipose tissue.
A glycoprotein derived from FBS that is used as Fetuin has been used at a concentration of 500 mg/ml to supplement serum free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells.
A glycoprotein derived from FBS that is used as Fetuin has been used at a concentration of 500 mg/ml to supplement serum free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells.
Applicazioni
Fetuin from fetal bovine serum has been used:
- as a substrate to determine neuraminidase activity
- to validate the specificity of sialidases
- to stabilize m-calpain
Azioni biochim/fisiol
Fetuin acts as an inhibitor of insulin receptor tyrosine kinase. It modulates bone remodeling and calcium metabolism. Fetuin regulates vascular calcification, insulin resistance, protease activity control, keratinocytes migration and breast tumor cell proliferative signaling. It functions as a biomarker for neurodegenerative diseases. Fetuin exhibits anti-inflammatory property.
Nota sulla preparazione
Prepared by ammonium sulfate fractionation of fetal bovine serum by the method of Pederson, K.O., J. Phys. and Colloid Chem., 51, 164 (1947).
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Mellgren RL and Huang X
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Dabrowska AM, et al.
Biomedical Papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 159(3), 352-359 (2015)
One-step affinity purification of fetuin from fetal bovine serum
Cartellieri S, et al.
Biotechnology and Applied Biochemistry, 35(2), 83-89 (2002)
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