F2055
Monoclonal Anti-hFBXW7
clone FB407, purified from hybridoma cell culture
Sinonimo/i:
Anti-ARCHIPELAGO (Drosophila), Anti-Cdc4 (S. serevisia), Anti-F-Box and WD40 Domain Protein 7, Anti-FBW7, Anti-SEL-10 (C. elegance)
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About This Item
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified from hybridoma cell culture
Tipo di anticorpo
primary antibodies
Clone
FB407, monoclonal
Stato
buffered aqueous solution
PM
antigen ~60 kDa
Reattività contro le specie
rat, human, mouse
Concentrazione
~2 mg/mL
tecniche
immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts
Isotipo
IgG2a
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... FBXW7(55294)
mouse ... Fbxw7(50754)
Descrizione generale
FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.
Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.
Specificità
The antibody recognizes human, mouse, and rat hFBXW7.
Immunogeno
synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.
Applicazioni
Anti-hFBXW7 antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Azioni biochim/fisiol
F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
The Fbxw7/hCdc4 tumor suppressor in human cancer
Tan YM, et al.
Cancer letters, 271(1), 1-12 (2008)
Markus Welcker et al.
The Journal of biological chemistry, 280(9), 7654-7658 (2004-12-22)
Cell transformation by simian virus 40 (SV40) results mostly from the highly oncogenic activities of the large T antigen (LT), which corrupts the cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. The most
Markus Welcker et al.
Current biology : CB, 14(20), 1852-1857 (2004-10-23)
The human tumor suppressor Fbw7/hCdc4 functions as a phosphoepitope-specific substrate recognition component of SCF ubiquitin ligases that catalyzes the ubiquitination of cyclin E , Notch , c-Jun and c-Myc . Fbw7 loss in cancer may thus have profound effects on
Harith Rajagopalan et al.
Nature, 428(6978), 77-81 (2004-03-06)
Aneuploidy, an abnormal chromosome number, has been recognized as a hallmark of human cancer for nearly a century; however, the mechanisms responsible for this abnormality have remained elusive. Here we report the identification of mutations in hCDC4 (also known as
Masayoshi Yada et al.
The EMBO journal, 23(10), 2116-2125 (2004-04-23)
The F-box protein Skp2 mediates c-Myc ubiquitylation by binding to the MB2 domain. However, the turnover of c-Myc is largely dependent on phosphorylation of threonine-58 and serine-62 in MB1, residues that are often mutated in cancer. We now show that
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