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Key Documents

F0926

Sigma-Aldrich

Siero fetale bovino

USDA approved, sterile-filtered, suitable for cell culture

Sinonimo/i:

FBS, FCS, sieri, siero

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About This Item

Numero MDL:
MFCD00132239
Codice UNSPSC:
12352207
NACRES:
NA.71

Origine biologica

bovine fetus

Livello qualitativo

500

Sterilità

sterile-filtered

Nome Commerciale

FBS Classic

Qualità

USDA approved

Composizione

Hemoglobin, ≤25 mg/dL

tecniche

cell culture | mammalian: suitable

Impurezze

≤10 EU/mL endotoxin

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Categorie correlate

Descrizione generale

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Applicazioni

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)

Compatibilità

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Risultati analitici

  • Testato per il contenuto in endotossine ed emoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

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